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Insect Cell Expression System

Baculovirus Expression Vector System (BEVS) is a kind of Eukaryotic expression system with high security. Due to a large genome so the BEVS can accommodate larger foreign gene, besides this, the recombinant viruses gained by BEVS can be easy to be screened. And it also has perfect post-translation modification system and efficient exogenous gene expression ability. BEVS is composed of transfer vector, baculovirus vector and host cells, which uses one or more baculovirus super promoter, and it can get recombinant baculovirus after the foreign target gene being inserted into the promoter. This recombinant baculovirus will replicate in insect cells and at the same time, the exogenous genes will get efficient expression.

Baculovirus Expression Vector System (BEVS) has the following advantages compared with other expression vector systems.
• Safety: Baculoviruses are essentially nonpathogenic to mammals and plants. They have a restricted host range, which is often limited to specific invertebrate species. Compared to other virus vectors, such as vaccinia virus and adenovirus vectors, BEVS has higher safety.
• Easy to amplification: Baculoviruses have been reproducibly scaled up for the large-scale production of biologically active recombinant products.
• High Levels of Recombinant Gene Expression: Compared to other Eukaryotic expression systems, the BEVS can efficiently express foreign protein from infected cells late in infection.

•High Biologically Activity of Recombinant Protein: Baculoviruses can be propagated in insect hosts and the recombinant protein gained by BEVS can have a posttranslational modification in a manner similar to the mammalian cells. Most importantly, the expression product has strong biological activity.

Insect cell expression system:
Restructuring for constructing physical grain
By use of mating-assisted genetic integrated cloning (MAGIC),we only need simple bacteria confusion, and then the recombinant baculoviruses vectors will be got rapidly, so a lot of time, energy and cost will be saved
30-50% off can be provided for renewal order
We can provide the detailed information of protein expression and purification, experimental data, as well as high titer of the virus liquid
Recombinant baculovirus carrier construction
Express conditions optimization
Protein expression and purification

Insect cells protein expression items
Test Test procedures Deliverable Timeline
1. protokaryon cloning ●Get gene
Nucleic acid electrophoresis images Two weeks

●PCR amplification product connected to pFastBac modified by our company
●Transform to E.coliDH10β

●Get correct recombinant donor plasmid
2. xtensification training ●Recombinant Baculoviruses obtain
●Recombinant Baculoviruses plasmid transfected insect cells
●High titer of the virus obtain

Plasmid DNA electrophoresis images with recombinant baculovirus vector,
Virus liquid with high titer

Three to four weeks
3. Optimization expression

●High titer of the virus liquid infects insect cells again
●Recombinant protein SDS-PAGE and western blot detection

SDS-PAG, western blot A half and one weeks
4. Protein expression and purification ●1L insect cells expression system and further purification 1L purified product of cell culture supernatant One to two weeks

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